1 、 AMV Reverse Transcriptase 5X Reaction Buffer: The AMV Reverse Transcriptase 5X Reaction Buffer supplied with this enzyme has a composition of 250mM Tris-HCl (pH 8.3 25°C ), 250mM KCl, 50mM MgCl 2 , 2.5mM spermidine and 50mM DTT.
2 、 Enzyme Storage Buffer: AMV Reverse Transcriptase (AMV-RT) is supplied in 200mM potassium phosphate (pH 7.2 4°C ), 0.2% Triton ? X-100, 2mM DTT and 50% glycerol.
3 、 Source: Purified from avian myeloblastosis virus particles.
4 、 Storage Conditions: Store at – 20°C . Avoid multiple freeze-thaw cycles and exposure to frequent temperature changes.
5 、 Unit Definition: One unit is defined as the amount of enzyme required to catalyze the transfer of 1nmol of deoxynucleotide into acid-precipitable material in 10 minutes at 37°C . The reaction conditions are: 50mM Tris-HCl (pH 8.3), 40mM KCl,
8.75mM MgCl 2 , 10mM DTT, 0.1mg/ml acetylated BSA, 1mM radiolabeled dTTP and 0.25mM poly(A):oligo(dT).
6 、 Usage Notes: The AMV Reverse Transcriptase 5X Reaction Buffer is intended for use in standard first-strand cDNA synthesis reactions. No deoxynucleotides are in the buffer; The formulation of AMV Reverse Transcriptase 5X Reaction Buffer is not compatible with M-MLV Reverse Transcriptase. Up to 10μl of an RT reaction containing AMV-RT and the supplied AMV Reverse Transcriptase Reaction Buffer can be added
to PCR amplification reactions that use TaqDNA Polymera |
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